In order to understand biological phenomena in cell systems, we need to determine the detailed mechanisms that express the functions of individual single protein molecules. This is not speculation from static structural information but time-resolved dynamic conformational ones from individual protein molecules. Recent progress in imaging the motions of individual single protein molecules in living cells has been achieved with several single-molecular techniques and systems by using visible light. In particular, single-molecule fluorescence resonance energy transfer (single-molecule FRET) is one of the few methods available for measuring nanometer-scale distance and the intermolecular orientation between two fluorophores. However, measuring intramolecular structural changes of single protein molecules with single-molecule FRET is very difficult due to the lack of both monitoring precision and stability of the signal intensity in physiological conditions. We proposed that one method for improving the positional decision accuracy is to shorten the light wavelength, e.g., x-rays, electrons, neutrons, and other accelerated ion probes (YC SASAKI, PRB 2000, PRL 2001, PRB 2004).
Recently, we succeeded in observing picometer-scale Brownian motions of individual membrane protein (bR, KcsA(Cell 2009), KvAP), antigen-antibody interactions, peptide/ MHC complex for T cell activation, ATP ligand protein (Myosin, Chaperonin) and monitoring super-weak force (pN) field with our single molecular detection system using x-rays, called Diffracted X-ray Tracking (DXT). We observed the rotating motions of individual nanocrystals, each of which was linked to a specific site in an individual protein molecule as shown in the following figure.
Operating mechanism analysis of biological nanomachines with one molecule measurement technique
Inorganic, microsecond X-ray time division observation of nano-sized molecular network of protein supersaturated solution
Structure fluctuation 1 molecule observation of α Shinukurei is the cause protein molecules of Parkinson’s disease
Observation of the catalytic action of the immune system molecules using X-ray single molecule tracking method
In 8th Nanomedeishin International Symposium, was announced following!
Shimura Masahiro’s M2 was awarded the Best Poster Award at a poster session.
“Anomalous Phosphorylated Tau Protein’s Dynamics from X-ray Single Molecule Observations”
In 37th Molecular Biology Society of Japan, it was announced following!
“One molecule dynamics measurement of tau protein denaturation natural area by the X-ray”
First time in the “3D active sites science” public workshop, it was announced following.
“The discovery of nano supersaturation 3D structure network by X-ray single molecule tracking method and its application to biomolecular systems”
In surface science academic lecture, we have been the following announcement!
“Crystallization precursor cluster dynamics observation using the X-ray single molecule tracking method and its interface properties evaluation”
“Dynamics Measurement natural denatured protein molecules immobilized on the surface.”
In Crystallographic Society of Japan Annual Meeting, we have been the following announcement!
“Evaluation of the physical properties of the precursor cluster of supersaturated solution of using the X-ray single molecule tracking method”
12th Science fusion visualization symposium was held!
Sasaki Kende Visualization and one molecule measurement method of the thermal fluctuation by dark-field microscope (Brownian motion) ( I was awarded the X-ray single molecule tracking method and Nobel Prize in Chemistry 2014Super-resolution microscope) I was introduced.
It was a precious time who can not let the talk with a variety of people!