In order to understand biological phenomena in cell systems, we need to determine the detailed mechanisms that express the functions of individual single protein molecules. This is not speculation from static structural information but time-resolved dynamic conformational ones from individual protein molecules. Recent progress in imaging the motions of individual single protein molecules in living cells has been achieved with several single-molecular techniques and systems by using visible light. In particular, single-molecule fluorescence resonance energy transfer (single-molecule FRET) is one of the few methods available for measuring nanometer-scale distance and the intermolecular orientation between two fluorophores. However, measuring intramolecular structural changes of single protein molecules with single-molecule FRET is very difficult due to the lack of both monitoring precision and stability of the signal intensity in physiological conditions. We proposed that one method for improving the positional decision accuracy is to shorten the light wavelength, e.g., x-rays, electrons, neutrons, and other accelerated ion probes (YC SASAKI, PRB 2000, PRL 2001, PRB 2004).
Recently, we succeeded in observing picometer-scale Brownian motions of individual membrane protein (bR, KcsA(Cell 2009), KvAP), antigen-antibody interactions, peptide/ MHC complex for T cell activation, ATP ligand protein (Myosin, Chaperonin) and monitoring super-weak force (pN) field with our single molecular detection system using x-rays, called Diffracted X-ray Tracking (DXT). We observed the rotating motions of individual nanocrystals, each of which was linked to a specific site in an individual protein molecule as shown in the following figure.

• 3rd bukkouken Science Festa, 6th MLF Symposium, at the 32nd PF symposium, I announced the following!
• This symposium poster http://imss-festa.kek.jp/2014/IMSS_SF2014_PosterA4.pdf
• Held time: March 17, 2015 (Tuesday), the 18th (water)
• Venue: Tsukuba International Congress Center (Epochal Tsukuba) Yubinbango305-0032 Tsukuba, Ibaraki Takezono 2-20-3
• Organized by: KEK Institute of Materials Structure Science, J-PARC Center, Science and Research Organization (CROSS), PF- user association, J-PARC / MLF user meeting

Functional analysis of from one molecule dynamics measurement using a quantum beam Functional Analysis from Dynamical Single Molecule Observations with Quantum Beams

Operating mechanism analysis of biological nanomachines with one molecule measurement technique

Inorganic, microsecond X-ray time division observation of nano-sized molecular network of protein supersaturated solution

Structure fluctuation 1 molecule observation of α Shinukurei is the cause protein molecules of Parkinson’s disease

Observation of the catalytic action of the immune system molecules using X-ray single molecule tracking method

In 8th Nanomedeishin International Symposium, was announced following!

＜12/8 postscript＞
Shimura Masahiro’s M2 was awarded the Best Poster Award at a poster session.

• held Date: December 4, 2014 (Thursday) to 6 (Saturday)
• Venue: Ehime University MinamiKa Memorial Hall
• Organizer: Japan Nanomedeishin Exchange Association

“Anomalous Phosphorylated Tau Protein’s Dynamics from X-ray Single Molecule Observations”

In 37th Molecular Biology Society of Japan, it was announced following!

• Dates: November 25 to 27 years 2014
• Venue: Pacifico Yokohama

“One molecule dynamics measurement of tau protein denaturation natural area by the X-ray”
https://confit.atlas.jp/guide/event/mbsj2014/subject/2P-0066/advanced

First time in the “3D active sites science” public workshop, it was announced following.

• Date: November 21 (Friday) to 22, 2014 (Saturday)
• location: CIVI Training Center Shin-Osaka East 6th floor E605 conference room of the Shin-Osaka
• http://www.civi-c.co.jp/access.html#higashi

“The discovery of nano supersaturation 3D structure network by X-ray single molecule tracking method and its application to biomolecular systems”

• Matsushita-kun was awarded Student Award!

In surface science academic lecture, we have been the following announcement!

• Dates: November 6, 2014 (Thursday) to 8 (Saturday)
• Location: Shimane Prefectural Industrial Community House (Kunibiki Messe)

“Crystallization precursor cluster dynamics observation using the X-ray single molecule tracking method and its interface properties evaluation”

“Dynamics Measurement natural denatured protein molecules immobilized on the surface.”

In Crystallographic Society of Japan Annual Meeting, we have been the following announcement!

• Dates: November 1, 2014 (Saturday) to three days (Mon)
• Venue: University of Tokyo, Faculty of Agriculture (Hongo campus Yayoi district)

“Evaluation of the physical properties of the precursor cluster of supersaturated solution of using the X-ray single molecule tracking method”

12th Science fusion visualization symposium was held!

• “Toward the next generation visualization”
• date October 31, 2014 (Friday) 10:00 ~
• venue University of Tokyo Graduate School of Frontier Sciences, infrastructure building second floor large lecture room
• http://www.k.u-tokyo.ac.jp/info/entry/1_entry60/

Sasaki Kende Visualization and one molecule measurement method of the thermal fluctuation by dark-field microscope (Brownian motion) ( I was awarded the X-ray single molecule tracking method and Nobel Prize in Chemistry 2014Super-resolution microscope) I was introduced.

It was a precious time who can not let the talk with a variety of people!

• http://ug-lab.blogspot.jp/

• Quest mind and curiosity – More familiar feel science ~
• http://www.kashiwa.u-tokyo.ac.jp/opc2014/

• Molecule to function fluctuates! ? ~ That ~ X-ray single molecule measurement push open the academic fusion
• http://www.k.u-tokyo.ac.jp/news/opc2014/kiban/contents/1.html